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An easy "SteamDrop" method for high quality plant chromosome preparation.pdf This Provisional PDF corresponds to the article as it appeared upon acceptance. Fully formatted

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The Determination of Short and Long Arms of 6D Chromosomes. To determine that the arm of 6D containing Oligo-6DS.1 signal is short or long arm, the consensus sequences of probe Oligo-6DS.1 was used for Nucleotide BLAST searches against the IWGSC RefSeq v1.0 of wheat Chinese Spring using blastn tool in B2DSC, and chromosome plots were drawn to view the locations of the consensus sequence on ...

Conclusion. The SteamDrop procedure provides a robust and routine method for high quality plant chromosome preparations. The method can be applied for metaphase as well as pachytene chromosome preparation in wide range of species.

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Plant nuclear genomes. The plant nuclear genome, consisting of the DNA and associated proteins, is organized into discrete chromosomes. Each unreplicated chromosome and metaphase chromatid consists of a single DNA molecule that is linear and unbroken from one end to the other ().

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